Transcriptomic study (RNAseq) of diurnal expression of Brachypodium distachyon ecotypes Bd21 and Bd21-3 under submergence stress

To study the diurnal transcriptome (RNA-seq) expressed by the monocot model plant B. distachyon under submergence stress, we analyzed two different ecotypes (Bd21, submergence sensitive; Bd21-3, submergence tolerant) at 5 different time points (dawn, ZT0; midday, ZT8; dusk, ZT16; middnight ZT20, end of night, ZT24). We studied mRNA expression in aerial plant tissue of submerged plants (S) and two types of controls: low light controls (LL) and normal growth controls (NG). All samples had 3 biological replicates. Each replicate consisted of a pool of 5 plants.