The P-body protein 4E-T regulates the balance between cell genesis and establishment of the postnatal neural stem cell pool by repressing mRNA translation [Ribotag]

To investigate the translatome of postnatal precursors of the V-SVZ cultured as neurospheres, we employed RiboTag analysis We performed RiboTag analysis on precursors derived from NestinCreERT2;RPL22HA/HA animals using specific anti-Hemagluttinin antibodies versus non-specific IgG controls Neurospheres were grown for 5 days, tamoxifen was added, and cells were lysed and collected 2.5 days later, immunoprecipitated with either anti-HA or anti-IgG, RNA was isolated and sequenced