Data underlying the publication: Quantitative physiology and proteomic adaptations of Bifidobacterium breve NRBB57 at near-zero growth rates
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https://data.4tu.nl/articles/_/21960575
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This data set corresponds to the proteome of <em>Bifidobacterium breve</em> NRBB57 which was cultivated at different growth rates from high (0.4 h-1) to near-zero growth rates. The bacteria was cultivated in retentostat and chemostat systems in a media with lactose as energy source. For the proteome analysis, samples were taken from the steady states of 3 biological replicates of the chemostats (µ=0.4 h-1, µ=0.12 h-1, µ=0.05 h-1, µ=0.025 h-1) and from 3 biological replicates of the retentostats after 1, 2 and 3 weeks after connecting the filter. Relative protein quantification was performed by Proteome Discoverer based on peptide intensity signals using default settings. <br>
本数据集对应短双歧杆菌(Bifidobacterium breve)NRBB57的蛋白质组,该菌株以0.4 h⁻¹至接近零的不同生长速率进行培养。实验采用以乳糖为能源的培养基,分别在恒化器(chemostat)与滞留培养系统(retentostat)中完成细菌培养。蛋白质组分析所用样品取自两类:一是4种生长速率(μ=0.4 h⁻¹、0.12 h⁻¹、0.05 h⁻¹、0.025 h⁻¹)下恒化器的3次生物学重复的稳态样品;二是滤器连接后第1、2、3周的滞留培养系统的3次生物学重复样品。相对蛋白质定量分析采用蛋白质组发现者(Proteome Discoverer)软件,基于肽段强度信号,以默认参数完成。
提供机构:
Mastrigt, van, Oscar; Abee, Tjakko; Smid, Eddy J.; Amor, Kaouther Ben
创建时间:
2023-02-06



