PSME3 Regulates Migration and Differentiation of Myoblasts [CUT&Run]

The acquisition of cellular identity requires large-scale alterations in cellular state. The non-canonical proteasome activator PSME3 is known to regulate diverse cellular processes, but its importance for differentiation remains unclear. Here, we demonstrate that PSME3 binds dynamically to highly active promoters over the course of differentiation. However, loss of PSME3 does not globally affect mRNA transcription. We find instead that PSME3 influences the levels of several adhesion-related proteins and acts upstream of the HSP90 co-chaperone NUDC to regulate cell motility and myoblast differentiation in a proteasome-independent manner. Our findings reveal several new facets of PSME3 functionality and highlight its particular importance for the differentiation of myogenic cells. Overall design: CUT&RUN was performed on C2C12 cells at two separate stages of serum deprivation-induced differentiation: prior to differentiation (cycling) and after 48 hours (day 2). CUT&RUN was performed using antibodies targeting the proteasome regulator PSME3, H3K4me3, or a control IgG