Adrenalectomy effect on muscle clock.

Characterisation the effect of removal of adreanl glands on skeletal muscle rhythmic transcriptome. Mice were anesthetized with 2% inhaled isoflurane and a midline incision were done in the abdominal cavity. The fat attached to the adrenal glands was pulled out of the cavity and after dissection of the adrenal gland, the fat was returned to the cavity, and the skin was sewn. The contralateral adrenal gland was similarly removed after two days. To compensate for the aldosterone loss, 0.9% of saline supplemented with 1 % glucose was provided. A sham-operated group was used as a control. Muscle samples were then taken from the animals after they had recovered for at least two weeks from the effects of the adrenalectomy and anesthesia. In order to treat adrenalectomized animals with exogenous hormones, an intraperitoneally (IP) injection of 5 mg/kg dexamethasone (Sigma # D1159) or 0.5 mg/kg adrenaline (Sigma #E4642) was given for 10 days at ZT12. Mice were than incubated for 2 weeks after treatment to recover from the effects of the adrenalectomy and anesthesia, before the collection of final muscle samples.