Expression data of butyrate stimulated epithelial organoid cultures generated from intestinal mucosa

In order to investigate the response of epithelium to butyrate, we generated in vitro epithelial organoid cultures from colon samples of non-IBD controls and they were stimulated with different doses of butyrate. The transcriptional signature revealed that butyrate negatively regulated proliferation and cell cycle, induced a protective response to oxidative stress and regulated genes related to the immune response. Colon samples from non-IBD controls (subjects undergoing surgery for colorectal cancer) were collected. Epithelial organoid cultures (EpOCs) were expanded from human colonic crypts and then induced to differentiate (d-EpOCs). Paired first-passage EpOCs and d-EpOCs (n=4) were stimulated with two doses of butyrate (0.5 mM and 5 mM) during 1h, 3h, 6h and 24h. RNA was then extracted and used for hybridization on Affymetrix microarrays.