Metagenomic assessment of the effect of sample processing on different microbiomes

Based on 4 samples processed differently (Pig feces 1 & 2 (P1 & P2) Sewage 1 & 2 (S1 & S2)), storage conditions (Time 0h, 16h, 64h Temperature (-80C, -20C, 5C, 22C)), sequencing platform (FTX=Freeze thawing, HX=Nextflex Hiseq, LTSX=Kappa Hiseq, NX1NS=Nextera Nextseq, NX2NS=Nextera Nextseq, NFNS=Nextflex Nextseq). Sample names containing HHKTMBBXX are resequenced samples. Sample names containing MC are spiked with a mock community. Sample names containing EC, ECPBS and pureMC, are negative DNA extraction controls, negative controls containing PBS and postive controls of the mock community used to spike with, respectively. The a, b and c are indicators of replicate number.