Paternal tobacco smoke correlated to offspring asthma and prenatal epigenetic programming

To investigate whether paternal tobacco smoke (PTS) was associated with prenatal epigenetic programming of CG site methylation, 20 cord blood DNA samples from newborns with and without PTS were subjected to microarray assay of 1,505 CG loci in 807 genes by Illumina GoldenGate® technology bead system to obtain high-throughput DNA Methylation profiles. Samples included 14 newborns without PTS exposure, and 6 newborns with PTS exposure. 20 umbilical cord blood samples were subjected to DNA extraction, following bisulfite conversion and hybridized to the Illumina GoldenGate Methylation Cancer Panel I array.