Lamins organize the global three-dimensional genome from the nuclear periphery

To visualize whether HiLands-P chromatin is decondensed, we used Oligopaint method -a modified fluorescence in situ hybridisation (FISH) technique specifically for imaging large Chromatic loci-to quantify four selected HiLands-P regions from chromosomes 1, 4, 13, and 14. After the hybridisation, we used Imaris software to quantify both the volumes and surface areas of these FISH signal in WT and TKO mESCs. The comparison demonstrated that both the volumes and surface areas of these regions increased significantly in TKO mESCs.