Lamins organize the global three-dimensional genome from the nuclear periphery

To visualize whether HiLands-P chromatin is decondensed, we used Oligopaint method -a modified fluorescence in situ hybridisation (FISH) technique specifically for imaging large Chromatic loci-to quantify four selected HiLands-P regions from chromosomes 1, 4, 13, and 14. After the hybridisation, we used Imaris software to quantify both the volumes and surface areas of these FISH signal in WT and TKO mESCs. The comparison demonstrated that both the volumes and surface areas of these regions increased significantly in TKO mESCs.为了探究HiLands-P染色质是否发生解凝集，我们采用Oligopaint技术——一种专为成像大型染色质位点设计的改良型荧光原位杂交（fluorescence in situ hybridisation, FISH）技术——对来自1、4、13和14号染色体的4个选定HiLands-P区域开展定量分析。杂交完成后，我们借助Imaris软件对野生型（Wild Type, WT）及三重敲除小鼠胚胎干细胞（TKO mESCs）中的这些FISH信号的体积与表面积进行定量检测。对比分析结果表明，TKO mESCs中上述区域的体积与表面积均显著升高。