Mangosteen Transcriptome and Transdecoder Files

Mangosteen Illumina RNA sequences were obtained from the NCBI Sequence Read Archive (SRA) databases. Trimmomatic 0.39 was used to remove adaptor sequences and low-quality reads from Illumina data with default parameter (TruSeq3-PE-2.fa:2:30:10 SLIDINGWINDOW:4:5 LEADING:5 TRAILING:5 MINLEN:25). Next, the clean Illumina data was used for de novo transcriptome assembly using Trinity v.2.9.1 with default parameters (adding the following parameter: normalize_by_read_set; min_kmer_cov=2; no bowtie) to generate a single Trinity assembly. After assembly, non-redundant genes/ reference transcriptome were obtained using TGICL with default settings. Next, Transdecoder was used to generate a total of 93,923 predicted peptides.