Multi-modal analysis of the aging mouse lung at cellular resolution

A) Whole lung tissue from 24 months (n=7) and 3 months old (n=8) mice was dissociated and single-cell mRNAseq libraries generated with Drop-Seq. B) Bulk RNA-seq data was generated from whole mouse lung tissue of old (n=3) and young (n=3) samples. C) Bulk RNA-seq data was generated from flow-sorted macrophages from old (n=7) and young (n=5) mice and flow-sorted epithelial cells from old (n=4) and young (n=4) mice.

A) 对24月龄（n=7）与3月龄（n=8）小鼠的全肺组织进行解离处理，并通过Drop-Seq构建单细胞mRNA测序文库。B) 以老年（n=3）与青年（n=3）小鼠的全肺组织为样本，生成批量RNA测序（bulk RNA-seq）数据。C) 分别以老年（n=7）、青年（n=5）小鼠的流式分选巨噬细胞，以及老年（n=4）、青年（n=4）小鼠的流式分选上皮细胞为样本，生成批量RNA测序数据。

搜集汇总

背景与挑战

背景概述

该数据集是一个多模态研究，结合单细胞和批量RNA测序技术，分析衰老小鼠肺在细胞分辨率层面的变化。数据集包含41个样本，覆盖老年（22个月）和年轻（3个月）小鼠的肺组织、上皮细胞和巨噬细胞，使用Illumina HiSeq 4000平台进行转录组测序，旨在揭示衰老相关的分子机制。