Path-seq identifies an essential mycolate remodeling program for mycobacterial adaptation in host cells [ChIP-seq]

Study of the DNA binding sites of the transcriptional regulator MSMEG_0916 through chromatin immunoprecipitation couple with deep sequenicng (ChIP-seq) and induced or uninduced overexpression of MSMEG_0916 in Mycobacterium smegmatis Cultures of Mycobacterium smegmatis with pDTCF-MSMEG_0916 were grown in the presence or absence of anhydrotetracycline (ATc) for 4 hour and DNA-protein interactions were characterized by cross-linking 50 ml of culture with 1% formaldehyde for 30 min. Immunoprecipitated DNA was sequenced aligned with Bowtie2 and processed using the R package DuffyNGS.