IL-27 regulates the differentiation of follicular helper NKT cells via metabolic adaptation of mitochondria

To investigate transciprtomic landscape changes of Gr-1+ cells after P/A immunization and NKT cells after anti-IL-27 antibody, we isolated Gr-1+ cells on day 1 and 3 after P/A immunization and NKT cells on day 3 after P/A immunization with anti-IL-27 antibody and isotype contorl. We then performed gene expression profiling analysis of RNA-seq data for Gr-1+ cells on day 0, 1, and 3 after P/A immunization and NKT cells on day 3 after P/A immunization with anti-IL-27 antibody and isotype control. (n=3 per group) Overall design: See "Materials and Methods".