Glucose deprivation regulates the expression of BACH1-upregulated pro-metastatic genes

Since the primary effect of the ketogenic diet is to establish a glucose-deprived condition in vivo, we, therefore, used glucose starvation in vitro to mimic the effects of the keto diet. In our endeavor to comprehend the influence of glucose starvation on pro-metastatic genes associated with BACH1, we performed RNA-Seq analysis on MDA-MB-231 ctrl cells and BACH1 knockout cells. MDA-MB-231 Ctrl cells (2 individual clones) and BACH1 knockout cells (3 individual clones) were incubated in DMEM medium with either 4.5g/L or 0g/L glucose for 40h. Subsequently, the cells were collected for RNA sequencing.