Triticum aestivum Raw sequence reads

The resistant DNA bulk and susceptible DNA bulk were performed by wheat exome capture sequencing. Briefly, DNA were fragmented and selected for an average insert size of 200 bp to construct the sample libraries. Hybridization of sample libraries was performed using a wheat exome capture probe set with an accumulated probe length of 245 Mb can cover 277 Mb gene regions considering the capture of the flanking sequences including promoter regions, untranslated regions neighboring to the exons, and introns. The hybrids were washed and amplified by ligation-mediated PCR. The libraries were sequenced using the Illumina HiSeq Nova platform, followed by standard paired-end 150 bp sequencing library construction protocols.