Species-specific detection of polar cod (Boreogadus saida) and saffron cod (Eleginus gracilis) eDNA in the Bering and Chukchi seas during the MR23-06C research cruise

To provide an overview of the distribution of polar cod (Boreogadus saida) and saffron cod (Eleginus gracilis) in the Bering and Chukchi Seas, eDNA samples collected during the MR 23-06C cruise, which conducted by the 'R/V Mirai' of Japan Agency for Marine-Earth Science and Technology, were analyzed. eDNA samples were collected by filtering surface seawater and seawater obtained from the middle or bottom layers. Filtration was performed using a cartridge filter (Sterivex HV, 0.45 m pore size; Millipore, Burlington, MA, USA). The DNA of both species was quantified in environmental DNA (eDNA) samples utilizing a species-specific TaqMan qPCR assay.为概述北极鳕（Boreogadus saida）与细身宽突鳕（Eleginus gracilis）在白令海和楚科奇海的分布情况，研究人员对由日本海洋地球科学技术厅（Japan Agency for Marine-Earth Science and Technology）的‘未来号’（R/V Mirai）科考船执行的MR 23-06C航次所采集的环境DNA（eDNA）样本进行了分析。本次航次通过过滤表层海水以及中层、底层海水采集环境DNA样本，过滤操作采用筒式过滤器（cartridge filter），具体为孔径0.45 μm的Sterivex HV滤膜（密理博Millipore，美国马萨诸塞州伯灵顿市）；研究人员利用物种特异性TaqMan实时荧光定量PCR（qPCR）测定法，对环境DNA样本中两种鱼类的DNA完成了定量检测。