Targeting Immune-Fibroblast Crosstalk in Myocardial Infarction and Cardiac Fibrosis II

To examine whether commonly used human cultured fibroblast systems model human cardiac fibroblasts, we tested three different fibroblast cell preparations: primary human cardiac fibroblasts (NHCF), human dermal fibroblasts (NHDF), and immortalized human cardiac fibroblasts (iHCF). Each fibroblast cell line was treated with vehicle, TGF-β, or IL-1β for 72 hours and scRNAseq performed. Multiple biological and technical replicates were included, individually hash tagged, and pooled to minimize any potential batch effects.