Chemical induction of hepatocyte revitalization in mouse hepatocytes [RNA-seq 1]

We then compared whole transcriptome bulk RNA-seq data of dHeps isolated from CCl4-injured mice and treated with either 5C, 3C, or DMSO. The expression of hepatocyte-enriched TFs and hepatocyte functional genes was upregulated in both the 5C and 3C groups, while mesenchymal markers (Acta2, Mmp3), inflammatory-related genes (Cxcl10, Ecm1, Ccl6, and Cd68), and oxidative stress-related genes (Ncf2 and Ncf1), were down-regulated compared with their expression in the DMSO control group Overall design: Comparative gene expression profiling analysis of RNA-seq data for damaged hepatocytes treated by 5C cocktail (i.e., Salidroside 5 µM, CID755673 50 µM, Forskolin 2 µM, GSK429286A 2 µM, and ETC-1002 5 µM), a 3C cocktail (i.e., CID755673 50 µM, GSK429286A 2 µM, and ETC-1002 5 µM), or the solvent control (DMSO) at day16.