RNA-seq transcriptional profiling in sciatic nerves from wildtype and Pmp22 transgenic rats during perinatal development

The goal of this study was to identify deregulated genes in Schwann cells of Pmp22 transgenic rats in comparison to wildtype rats. Three timepoints in the course of peripheral nerve myelination were chosen (embryonic day [E] 21, perinatal day [P]6 and P18) in order to reveal mechanistic insight into early pathological processes of Charcot-Marie-Tooth disease 1A (CMT1A). Overall design: Three perinatal timepoints were chosen (E21, P6, P18) in order to cover the course of peripheral nerve myelination. The sciatic nerves of wildtype and Pmp22 transgenic rats were collected in four biological replicates per timepoint and genotype.