RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with fructooligosaccharide. RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with fructooligosaccharide

Purpose: Our data significantly advance understanding of Fructooligosaccharide regulatory mechanism in ileum of Taiping chicken Methods: Using RNA-seq analysis to study the gene expression in ileum tissue after Taiping chicken was given Fructooligosaccharide Results: A total of 164,629,826 and 152,047,552 raw reads were generated from the CT and FOS, respectively . After removing the interference data, about 164,028,872 and 151,452,588 clean reads were obtained Conclusions: through high-throughput sequencing of the six libraries from ileum of Taiping chicken, the expression level of mRNA has significant changes after given probiotics Overall design: Control group (CT), birds were fed a corn-soybean basal diet , Fructooligosaccharide group (FOS), birds were given a basal diet supplemented with 1 mL of Fructooligosaccharide per litre of water