Anti-IgE Effect of Naturally Occurring Arctigenin on Food Allergy Association with a Distinct Transcriptome Profile

Purpose: To identify IgE inhibitory bioactive compound from A.Lappa for targeted therapy on peanut specific IgE production and hypersensitivity reactions, and to investigate its underlying mechanisms. The goals of this study were to compare the transcriptome map (RNA-seq) and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) before and after PMBC in patients with food allergy, and to evaluate the most critical genes and signaling pathways which inhibit IgE production by arctigenin. Overall design: IgE inhibitory effects on human B cells (U266 human myeloma cells) and peripheral blood mononuclear cells (PBMCs) from food allergic patients, and in peanut sensitized and challenged murine model were assessed. And genes involved in IgE inhibition were studied with transcriptome sequencing. Examination of 3 set of transcriptome profiles of PBMCs (control, IL-4/anti-CD40 stimulation, IL-4/anti-CD40 stimulation+arctigenin).