Rap1a GTPase mVSM Knockout vs. Rescue mRNA Expression

Transcriptional profiling of mouse tail artery explanted microvascular smooth muscle (mVSM) cells comparing Rap1a -/- mVSM cells transduced with vector backbone (control) with Rap1a -/- mVSM cells transduced with a Rap1 CA 'rescue' plasmid. The experiment will compare mRNA expression in Rap1A -/- mVSMs and 'rescued'with Rap1A CA. Two-condition experiment, mVSM Rap1a -/- (vector control) vs. mVSM-RapCA. Biological replicates: 4 control replicates, 4 transduced replicates.