Parvovirus Minute Virus of Mice Localizes to Sites of Cellular DNA Damage to Establish and Amplify its Lytic Infection

We have developed a generally adaptable, novel high-throughput chromosome conformation capture assay for use in trans (V3C-seq) that allows genome-wide identification of the direct associations of a lytic virus genome with discreet regions of the cellular chromosome. Upon infection, the parvovirus Minute Virus of Mice genome associated directly with sites of cellular DNA damage. These sites also exhibited damage in uninfected cells when cycling through S-phase. As infection proceeded, new sites of DNA damage were induced, and virus subsequently also associated with these. Overall design: Identification of cellular interaction site of the parvovirus MVM, and the localization of the resultant DNA damage response. Independent replicates of chromosome conformation capture (designated as V3C in the file name) and ChIP-seq experiments (the target epitope provided in the name) have been performed. For each time-point or treatment, at least two independent replicates of the indicated assays have been performed. ChIP-seq assays have been normalized to total input DNA prior to rpm normalization. Chromosome conformation capture assays have been rpm normalized. A total number of twenty three samples have been deposited. "hpt" designates hours post treatment with hydroxyurea, "hpr" designates hours post release from cell cycle synchronization and "hpi" designates hours post infection with the parvovirus Minute Virus of Mice.