Detection of integrated HIV-1 genome in epithelial cells by qPCR.
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https://figshare.com/articles/dataset/_Detection_of_integrated_HIV_1_genome_in_epithelial_cells_by_qPCR_/1035573
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aC8166 cells express CXCR4 and were used for X4 viral infections only.
bNP2-R5 cells express CCR5 and were used for R5 viral infections only.
+, Integrated HIV-1 product detected (cycle threshold detection in brackets).
ND, Integrated HIV-1 product not detected.
Real-time PCR assay to detect integrated HIV-DNA using HIV-1 LTR and human Alu-specific primers and a U5 specific probe [92]. TR146, FaDu, A431 and PM-1 (control) cells were exposed to YU2 (R5) or LAI (X4) virus for 48 h at 37°C. DNA was extracted, digested with DpnI to degrade any plasmid DNA contaminant and analysed by real-time PCR.
创建时间：
2014-05-23
