Difference analysis of microRNAs in peripheral blood of asthmatic children by high-throughput sequencing

Asthma is the most common chronic pulmonary disease in children. The pathogenesis of asthma is not fully understood,and existing treatments have little effect on some children.MicroRNAs(miRNAs) play a regulatory role in the occurrence and development of asthma,and miRNA therapy is a promising treatment for asthma.The purpose of this study was to explore the differential expression of miRNAs in peripheral blood of children with asthma and to find a miRNA that can alleviate asthma inflammation.We used high-throughput sequencing to analyze miRNA differences in peripheral blood between children with acute asthma attacks and healthy children,target gene prediction and functional enrichment analysis were performed for them..Overall,51 differentially expressed miRNAs were identified,with 9 being upregulated and 42 downregulated. To explore the differences in miRNA in peripheral blood of children with asthma and healthy children, we collected venous blood samples of children with asthma and healthy children, extracted total RNA, and analyzed them using high-throughput sequencing.