Jurkat cell exosomes bound by CD47, CD63 and MHC1 harbor different RNA content. Homo sapiens

Extracellular vesicles (EVs) mediate cell-cell communication including the intercellular transfer of RNAs, which alter gene expression in target cells. However, heterogeneity in the size, density, and composition of EVs has limited progress towards defining their physiological functions and utility as disease-specific biomarkers. CD63 and MHC-1 are widely used as markers to purify EVs. CD47 is also present on EVs and alters their effects on target cells, suggesting that specific surface markers define functionally distinct EVs. This hypothesis was addressed by comparing the properties of Jurkat T cell EVs captured using CD47, CD63, and MHC-1 antibodies. These EV subsets have similar sizes but divergent RNA contents. Overall design: Jurkat cell exosomes where bound by CD47, CD63 and MHC1 antibodies and compared to their un-bound exosomes using a total of 14 samples: triplicates for CD47 bound and unbound and duplicates for the rest.