Genome-wide essential gene identification for Shiga toxin-induced cell death using CRISPR/CAS9 screening system with Vero cells

Isolation of genes in Vero cell whose knockout confer resistance to Stx-induced cell death A genome-wide CRISPR/CAS9 knockout screen in Vero C1008 cells was performed to identify host factors involved in the regulation of glycan biosynthesis by exploiting Stx sensitivity.