Host and Viral Genes Regulated in λ Lysogens

Columns A, B, C, and D are the spot coordinates; Column E: The E. coli gene ID number of the original ORFmer primer set; Column F: gene name (λ genes are prefixed with “LMD”); Column G: M = log2 of the expression level ratio of lysogen to nonlysogen (see also Figure 2 caption); Column H: A = log [(channel 1)(channel 2)]1/2, a measure of the brightness of the fluorescent signal; Columns I, J, and K: Statistic based on the pooled data from 48 individual measurements using 12 different samples taken from exponentially growing cells. The E. coli MG1655 primer set from Sigma-Genosys (Woodlands, Texas, United States) was designed to amplify 4,539 open reading frames and is therefore very heterogeneous in target length. The λ set of 73 primer pairs contained some duplicate targets, for example, LMD__rexA__L and LMD__rexA in which the L set was approximately 300 nucleotides long and the other set contained approximately 70 nucleotides. The LMD primer set is available from the corresponding author (E-mail: [e-mail]. (764 KB XLS).