A. thaliana cycd3;1-3 leaf8 time-lapse images
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Raw confocal stacks used in the following Manuscript: TITLE: Cell-cycle-linked growth reprogramming encodes developmental time into leaf morphogenesis JOURNAL: Current Biology Article Type: Research Article Authors: Xin-Min Li, Hannah Jenke, Sören Strauss, Christos Bazakos, Gabriella Mosca, Rena Lymbouridou, Daniel Kierzkowski, Ulla Neumann, Purva Naik, Peter Huijser, Stefan Laurent, Richard S. Smith, Adam Runions, Miltos Tsiantis* *Corresponding Authors: Miltos Tsiantis (tsiantis@mpipz.mpg.de) Leaf8 development in A.thaliana cycd3;1-3 triple mutant was tracked using live imaging with a 24-hour interval for 5-6 days (D, days after initiation). Four biological replicates are archived. tdTomato signals from the plasma membrane were captured by a Leica TCS SP8 Confocal Laser Scanning Microscope, and images were channel-split and converted into .tif format stacks using FIJI. For further information, please refer to the related publication.
本数据集为下述论文中所使用的原始共聚焦图像堆叠文件: 论文标题:《与细胞周期相关的生长重编程将发育时间编码于叶片形态发生之中》 期刊:《当代生物学》(Current Biology) 文章类型:研究论文(Research Article) 作者:李新民(Xin-Min Li)、汉娜·扬克(Hannah Jenke)、瑟伦·施特劳斯(Sören Strauss)、克里斯托斯·巴扎科斯(Christos Bazakos)、加布里埃拉·莫斯卡(Gabriella Mosca)、蕾娜·林布里杜(Rena Lymbouridou)、丹尼尔·基尔兹科夫斯基(Daniel Kierzkowski)、乌拉·诺依曼(Ulla Neumann)、普尔瓦·奈克(Purva Naik)、彼得·惠泽尔(Peter Huijser)、斯特凡·洛朗(Stefan Laurent)、理查德·S·史密斯(Richard S. Smith)、亚当·鲁尼恩斯(Adam Runions)、米尔托斯·齐安蒂斯(Miltos Tsiantis)* *通讯作者:米尔托斯·齐安蒂斯(邮箱:tsiantis@mpipz.mpg.de) 本研究以拟南芥(Arabidopsis thaliana)cycd3;1-3三重突变体为材料,对其第8片叶(Leaf8)的发育过程进行活细胞成像(live imaging)追踪,每24小时采集一次图像,持续5至6天(D,即起始后天数)。本数据集共归档4个生物学重复样本。 研究使用徕卡TCS SP8共聚焦激光扫描显微镜(Leica TCS SP8 Confocal Laser Scanning Microscope)采集质膜的tdTomato荧光信号,随后通过FIJI软件对图像进行通道分离,并转换为.tif格式的图像堆叠文件。如需获取更多详细信息,请参阅相关研究论文。




