The Tug1 locus is required for male fertility and comprised of DNA, RNA and Protein regulatory elements

We have sequenced mouse embryonic fibroblasts (MEFs) and 6 organs (testes, prostate, liver, heart, spleen, and eye) harvested from adult male wild type and Tug1 knockout (Tug1_tm1.1Vlcg) mice in a C57BL/6J/129S6 (N3-C57BL/6J, no Neo) background. Additional sequencing was done on the testes of a Tug1 rescue mouse, which contains a doxycycline inducible allele for Tug1 (tg(Tug1)) and CAGs-rtTA3 in the Tug1 knockout background (Tug1_tmn1.1Vlcg). Comparison between wild type and Tug1 knockout (Tug1_tm1.1Vlcg) organs and primary cell transcriptomes. Additional comparisons of the transcriptomes of wildtype, knockout, and rescue testes.