Transcriptome profiling of FK866 response in CCRF-CEM parental and resistant cells

Parental and resistant CCRF-CEM cells were treated for 48 hr with a sublethal dose of FK866 (5 nM) or DMSO (Mock, control treatment). This comparison enables the identification of the specific transcriptional alterations induced by FK866 in parental or resistant cells. Keywords: transcriptome profiling, FK866, drug resistance Gene expression signals derived from parental and resistant CCRF-CEM cells after FK866 treatment were compared by microarrays analysis. Total RNA were isolated from CCRF-CEM cells treated with FK866 5 nM or DMSO (mock, control treatment) for 48 hr. Cells lysates were collected from control cells (mock) and from treated cells (FK866). All experiments were run with biological replicates.