Relationship between experimental <i>ΔΔG</i>, Δlog₁₀(<i>k_off</i>), Δlog₁₀(<i>k_on</i>) and change in interface hotspot energy (<i>Int_HS_Energy</i>) for 713 mutations in SKEMPI.

(A) Shows PCC between experimental ΔΔG with the respective Δlog10(koff) and Δlog10(kon) for single-point alanine, single-point non-alanine, multi-point and all 713 mutations. (B) Shows PCC between Int_HS_Energy with the respective ΔΔG, Δlog10(koff) and Δlog10(kon) for single-point alanine, single-point non-alanine, multi-point and all 713 mutations. Experimental values for the 713 mutations used here are extracted from SKEMPI [41] and are presented in Dataset S1.