Oryza sativa cultivar:Japonica and oss40-14 mutant gene expression under dark condition

OsS40-14 is a member of the S40 family, playing a crucial role in dark-induced leaf senescence in rice. The oss40-14 knockout mutants exhibited a stay-green phenotype in both primary and flag leaves under dark-induced conditions, showing substantial retention of chlorophylls and photosynthetic capacity, along with remarkably reduced reactive oxygen species (ROS). However, the molecular mechanisms underlying dark-induced senescence and the key regulatory genes for specific biological processes remain poorly understood.