CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM RALSTONIA EUTROPHA H16 (H16_A1328), TARGET EFI-510189, WITH BOUND (S)-2-hydroxy-2-methyl-3-oxobutanoate ((S)-2-Acetolactate)

CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM RALSTONIA EUTROPHA H16 (H16_A1328), TARGET EFI-510189, WITH BOUND (S)-2-hydroxy-2-methyl-3-oxobutanoate ((S)-2-Acetolactate) Descriptor: (2S)-2-hydroxy-2-methyl-3-oxobutanoic acid, CHLORIDE ION, TRAP-type transporter, ... Authors: Vetting, M.W, Al Obaidi, N.F, Morisco, L.L, Wasserman, S.R, Stead, M, Attonito, J.D, Scott Glenn, A, Chowdhury, S, Evans, B, Hillerich, B, Love, J, Seidel, R.D, Whalen, K.L, Gerlt, J.A, Almo, S.C, Enzyme Function Initiative (EFI) Deposit date: 2014-03-31 Release date: 2014-05-07 Last modified: 2024-10-23 Method: X-RAY DIFFRACTION (1.3 Å) Cite: Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes. Biochemistry, 54, 2015

真养罗尔斯通氏菌H16（Ralstonia eutropha H16，基因座编号H16_A1328）来源的TRAP型周质溶质结合蛋白的晶体结构，结合配体为(S)-2-羟基-2-甲基-3-氧代丁酸[(S)-2-乙酰乳酸]，靶点编号EFI-510189。相关描述：(2S)-2-羟基-2-甲基-3-氧代羧酸、氯离子、TRAP型转运蛋白（TRAP-type transporter）等。作者：Vetting, M.W.、Al Obaidi, N.F.、Morisco, L.L.、Wasserman, S.R.、Stead, M.、Attonito, J.D.、Scott Glenn, A.、Chowdhury, S.、Evans, B.、Hillerich, B.、Love, J.、Seidel, R.D.、Whalen, K.L.、Gerlt, J.A.、Almo, S.C.、酶功能倡议（Enzyme Function Initiative, EFI）。提交日期：2014-03-31；发布日期：2014-05-07；最后修改日期：2024-10-23。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率1.3埃（Å）。引用文献：《用于功能注释与代谢物发现的实验策略：溶质结合蛋白靶向筛选与代谢组无差别淘选》，《生物化学（Biochemistry）》，第54卷，2015年。