The functional enhancer repertoire of human embryonic stem cells [STARR-RNA-seq]

We combined Self-Transcribing Active Regulatory Region Sequencing (STARR-seq) with an enrichment step using chromatin immunoprecipitation in a massively parallel reporter assay. We applied this assay, termed ChIP-STARR-seq, to normal (primed) and naive human embryonic stem cells, building up a comprehensive catalogue of functional enhancers. This database record describes the STARR-RNA-seq component. Overall design: For each ChIP-seq and BAC library: STARR-RNA-seq datasets after transfection into primed and/or naive hESCs = 42 datasets total