Figure 6. MDA5-producing species aligned fasta file

Nucleotide sequences for all MDA5-producing species were obtained from UniProt and aligned using AliView. AliView identified 83 stop codons in the human sequence, which were removed using a Python script. The script identified the first occurrence of the start codon ATG and adjusted sequences to begin at this position. The script ensured that the sequence length is a multiple of three, preserving the reading frame. Stop codons (TAA, TAG, and TGA) were replaced with "---", while headers from the original FASTA file were retained. The processed sequences were saved to a new FASTA file.