Selective T cell response of vaccinated mice.
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a Number of IFNγ secreting cells/106 splenocytes (splenocytes were removed 14 days following the last immunization (see Materials and Methods)). Data represents mean and SD from 3 individual animals (per group) derived from 3 independent experiments. b 107 CFU/ml of formalin-inactivated LVS (see Materials and Methods). c 10 µg of anti-CD4 antibodies were added to 106 splenocytes 1 hour before stimulation with formalin inactivated LVS. d 10 µg of anti-CD8 antibodies were added to 106 splenocytes 1 hour before stimulation with formalin inactivated LVS. e No-Ag, samples were tested without any added antigen. f mice were immunized by gene gun. (See Materials and Methods).
创建时间:
2014-01-06



