Spontaneous astrocytic calcium traces from organotypic hippocampal slices

Each column of the file (ROI) corresponds to an individual calcium trace, expressed as ΔF/F0.

Recordings were performed using confocal microscopy on organotypic hippocampal slices (Gähwiler type), with a spatial resolution around 175 nm (in x-y) and 450 nm (z). Confocal time-lapse imaging (12.5 x 25 μm, pixel size 100 nm) was performed at 2Hz. For more details, see the associated paper: Simulation of calcium signaling in fine astrocytic processes: effect of spatial properties on spontaneous activity, Denizot et al, PLOS Computational Biology, 2019.