Effect of depletion of USF1 or USF2 on Jurkat Tat mHIV-Luciferase T cell gene expression.

To investigate the cooperative function USF factors for the regulation of T cell transcriptom, we established Jurkat mHIV-Luciferase cells in which USF1 or USF2 was knocked out by CRISPR-Cas9. We then performed gene expression profiling analysis using data obtained from RNA-seq of cell lines either left untreated or stimulated by PMA and Ionomycin co-treatment. Overall design: Comparative gene expression profiling analysis of RNA-seq data for wildtype, USF1 KO, and USF2 KO cell lines under basal or activated conditions.