Mus musculus Raw sequence reads

The structure of the eukaryotic mitotic chromosome is one of the oldest unresolved problems in biology. Yet basic information necessary to understand its structure and assembly, such as its composition, is still lacking. In this study, using an optimized method to isolate metaphase chromosomes and 5'-tag sequencing, we characterize the mammalian mitotic chromosome-associated RNA (mCAR) population. Overall, the mCARs identified here, together with previously obtained proteomic and genomic data, constitute the first comprehensive catalogue of the molecular composition of the eukaryotic mitotic chromosomes.