Supplementary dataset to "A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses"

SUPPLEMENTARY DATASETS TO: <strong>A new genome allows the identification of genes associated with natural variation in aluminium tolerance in <em>Brachiaria </em>grasses</strong> <strong>Supplementary File 1:</strong> Cumulative root length (RL), root biomass (RB), and root tip diameter (RD) during Al³⁺ stress (A) and control (C) conditions, and the ratio (R) between stress and control values, in the interspecific progeny between CIAT 606 and BXR 44-02. <strong>Supplementary File 2: </strong>Gene annotation in GFF3 format. <strong>Supplementary File 3: </strong>Functional annotation of the genes, including GO terms and homologous proteins in NCBI nr database, Uniprot, <em>A. thaliana</em>, rice, <em>P. halli</em>, <em>S. italica</em> and <em>S. viridis</em><em>.</em> <strong>Supplementary File 4: </strong>Assignment of the proteins in the Poaceae family to eggNOG orthologous groups to identify shared clusters of proteins among these species. <strong>Supplementary File 5:</strong> Anchoring 21,145 <em>Brachiaria ruziziensis</em> scaffolds longer than 10 Kbp or with at least one annotated gene (533.9 Mbp) in <em>S. italica </em>nine chromosomes. <strong>Supplementary File 6:</strong> Chromosomal position of the 41,974 transcripts in <em>Brachiaria ruziziensis</em> based on the synteny with the <em>S. italica</em> genome. In BED5 format. <strong>Supplementary File 7: </strong>Genetic map with 4,427 markers placed at LOD 10 in 18 linkage groups, including the position of each marker in the genetic map and genome assembly. <strong>Supplementary File 8:</strong> Functional annotation of the 84 DE genes within QTLs. <strong>Supplementary File 9:</strong> Enrichment analysis of the GO terms (full ontology) over-represented among DE genes in each species with the biological processes (BP) and molecular functions (MF). <strong>Supplementary File 10:</strong> Enrichment analysis of the GO SLIM terms (reduced ontology) over-represented among DE genes in each species with the biological processes (BP) and molecular functions (MF). Margaret Worthington^1#, Juan Guillermo Perez¹, Saule Mussurova², Alexander Silva-Cordoba¹, Valheria Castiblanco¹, Juan Andres Cardoso Arango¹, Charlotte Jones³, Narcis Fernandez-Fuentes³, Leif Skot³, Sarah Dyer^2&amp;, Joe Tohme¹, Federica Di Palma², Jacobo Arango¹, Ian Armstead³, Jose J De Vega² 1. International Center for Tropical Agriculture (CIAT), A.A. 6713, Cali, Colombia. 2. Earlham Institute, Norwich Research Park, Norwich, NR4 7UZ, UK. 3. Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, UK.