Gene expression profile in the cuprizone-induced demyelination mouse model

Myelin abnormalities, oligodendrocyte damage, and concomitant glia activation are common in demyelinating diseases of the central nervous system (CNS). The neurotoxicant cuprizone (CPZ) has been extensively used to create a mouse model of demyelination. However, the changes of miRNA expression and effects on behavior of cuprizone treatment have not been clearly reported. We have analyzed the behavioral changes of mice given a diet containing 0.2% cuprizone for 6 weeks. To evaluate the changes of gene expression in CPZ-induced demyelination compared with control, we performed the GeneChip® mouse Gene 2.0 ST Array. We analyzed cerebellum from 2 subjects using the GeneChip® Mouse Gene 2.0 ST Array platform. Array data export processing and analysis were performed using Affymetrix® GeneChip Command Console® Software (AGCC).