RNA-Sequencing of EC and PVC from aged APOE mice. Mus musculus

RNA-Sequencing of entorhinal cortex and primary visual cortex from 14-15 month old APOE3/4 vs. APOE3/3 targeted replacement mice. Overall design: Entorhinal cortex and primary visual cortex tissue from 14-15 month old APOE mice (10 male APOE3/3s and 19 male APOE3/4s) were collected, and RNA was extracted using Trizol. Starting with 2 μg of total RNA per sample, Poly(A)+ mRNA was purified, fragmented and then converted into cDNA using the TruSeq RNA Sample Prep Kit v2. For RNA-Sequencing of the cDNA, we hybridized 5 pM of each library to a flow cell, with a single lane for each sample, and we used an Illumina cluster station for cluster generation. We then generated 149bp single end sequences using an Illumina Hi-Seq sequencer.