miRNA expression profiles in non-aggressive, invasive and metastatic papillary thyroid cancer

Paillary thyroid cancer (PTC) is the most common type of thyroid malignancy. Extrathyroidal invasion (ETİ), lymph node metastasis, and distance organ metastasis is poor prognostic factor in PTC. The metastasis is still a leading cause of papillary thyroid cancer death. The early detection of metastatic signature is crucial for identification of thyroid cancer prognosis and personalized therapeutic strategies. In the present study, we present thyroid cancer metastasis and invasivenes related miRNAs identified by comprehensive miRNA expression profiling of formalin-fixed paraffin embedded (FFPE) thyroid tissues obtained from patients belonging to intrathyroidal, invasive and metastatic thyroid carcinoma groups The formalin-fixed paraffin embedded (FFPE) carcinoma tissues of PTC patients from three different groups were included in the study: (i) non-aggressive tumors patients having carcinoma size <2 cm, intrathyroidal, with no lymphovascular invasion (group A, n=3); (ii) patients with minimal invasion of extrathyroidal soft tissue (group B, n=3); (iii) patients with distant organ metastasis (group C, n=3). FFPE tissues of the follow-up and treatment patients of the Kocaeli University Faculty of Medicine Endocrinology and Metabolism Clinic, between the years of 2003-2016, were obtained from the pathology archive of Kocaeli University Faculty of Medicine. Tumor histopathologic features such as histological subtype, extrathyroidal invasion, vascular and perineural invasion were evaluated by a clinopathological outcome blind pathologist. Groups were similar in age and gender.