Glycyrrhizic acid enhances mitochondrial autophagy by activating the BNIP3/Beclin-1 pathway Relieve colonic damage caused by cisplatin

This dataset contains the raw and processed data supporting the findings of the study titled: *"Glycyrrhizic acid enhances mitochondrial autophagy by activating the BNIP3/Beclin-1 pathway to relieve colonic damage caused by cisplatin"* (Running title: Glycyrrhizic acid enhances mitochondrial autophagy). The data were derived from an in vivo mouse model of cisplatin-induced colonic injury, investigating the protective effects of Glycyrrhizic acid (GA) alone or in combination with Urolithin A (UA). The dataset includes the following original and analyzed files: Histopathology: Hematoxylin and eosin (HE) staining images of colon tissues from all experimental groups (Control, Cisplatin, Cisplatin+GA, Cisplatin+GA+UA). Ultrastructural analysis: Transmission electron microscopy (TEM) images showing mitochondrial morphology in colonic epithelial cells. Apoptosis assessment: TUNEL staining fluorescence images and quantitative apoptotic index data. Immunohistochemistry (IHC): Original images and quantified integrated optical density (IOD) values for BNIP3, Beclin-1, p62, and LC3 protein expression in colon tissue. Protein expression (Western blot): Raw blots and densitometric quantification (including LC3-II/LC3-I ratios) for BNIP3, Beclin-1, p62, LC3, cleaved caspase-9, Bax, Bcl-2, and β-actin. Gene expression (qRT-PCR): Raw cycle threshold (Ct) values and relative expression levels (2⁻ΔΔCt) for BNIP3, Beclin-1, LC3, p62, Caspase9, Bax, Bcl-2, and β-actin. Oxidative stress markers: Raw absorbance and calculated values for malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPX) in colon tissue homogenates. Inflammatory cytokines: Serum concentrations of IL-6, IL-1β, and TNF-α determined by ELISA. Correlation analysis: Raw data and matrices used for Pearson correlation analysis between mitophagy-related proteins and apoptotic markers (C-caspase9).