Summary of the biological properties of pea eIF4E mutants.

aMutation found in the sbm1 resistance allele of pea eIF4E.bAmino acid position involved in binding m7GTP in the pea eIF4E crystal structure.ND = no data, NA = not applicable.In the PSbMV infection complementation assay, eIF4E variants were classified as having a ‘susceptible-like’ (S), ‘partially-susceptible’ (S*) or ‘resistant-like’ (R) phenotype. The support of yeast translation was scored as full-growth (++), reduced growth (+) and abolished growth (−), relative to eIF4ES. Conservation scores represent the degree of amino acid conservation ranging from variable (1) to conserved (9) calculated for 68 non-redundant plant eIF4E sequences.