Pseudomonas aeruginosa CLJ3 genome sequencing, transcriptomics, and proteomics [transcriptomics]

Gene expression profiles of two Pseudomonas aeruginosa taxonomic outlier clinical isolates, CLJ1 and CLJ3 [CLJ3] Pseudomonas aeruginosa taxonomic outliers emerged recently as infectious for humans, provoking hemorrhagic pneumonia. Those bacteria lack classical type III secretion system, and utilize the pore-forming toxin for infection. Two clones CLJ1 and CLJ3 belonging to these taxonomic outliers have been isolated from the same patient at two different times during hospitalization. P. aeruginosa CLJ3 displays antibiotic resistance phenotype, while CLJ1 is more cytotoxic on epithelial and endothelial cells. Total RNA was isolated from CLJ3 and sequenced in duplicate using Illumina HiSeq to compare their genome-wide transcription levels and relate these to protein levels and genome sequence.