Transcriptional dynamics of the murine heart during perinatal development

Analysis files related to the publication "Transcriptional dynamics of the murine heart during perinatal development at single-cell resolution" (Feulner et. al). Data was derived from a single-cell RNA-seq (Drop-seq) dataset of mouse hearts covering E14.5, E16.5, E18.5, P0, P4 and P7 timepoints. Relevant codes and list of R package versions are hosted at https://github.com/larafeulner/Perinatal_Mouse_Heart_scRNAseq.

FilteredCounts: QC-filtered raw counts for each timepoint replicate (in 10x format for easy importing in R or Python). These are the cell barcodes used for the final clustering.

Perinatal_clusterannot.Rds: Seurat object containing final clustering and annotation corresponding to Figure 1. 

subset_mats_psupertime: raw counts for main cardiac cell types (in 10x format) following subsetting and reclustering of Perinatal_clusterannot Seurat object, to be used for downstream analyses including psupertime. See Subclustering.Rmd on GitHub for example of generation.

subset_meta_psupertime: Corresponding metadata for subset_mats_psupertime.

psupertime_results: psupertime analysis output files for main cardiac cell types in .Rds format.