Raw dataset of light control effects on semen proteomic of the red-winged tinamou
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These data show profile proteomic of sperm and seminal plasma of red-winged tinamou (Rhynchotus rufescens) under the effects of light (natural light for controls versus 16 hours of light and 8 hours of darkness in treated birds), during of reproductive season. 20 males were randomly allocated to be Control (n = 10) or Artificial light (n = 10) groups. To sperm proteomics was used one sample from 10 birds (6 Control and 4 Artificial light groups) and for proteomics of seminal plasma were used two pools prepared with same samples from Artificial light group, the samples of the Control group had an insufficient volume to perform the proteomics. For this reason, the proteomics of seminal plasma was descriptive. Sperm and seminal plasma samples were individually prepared for proteomics (ESI Q-TOF MS/MS).
本数据集呈现了繁殖季节中红翅䳍(Rhynchotus rufescens)精子与精浆的蛋白质组学谱图,探究光照条件对其的影响:对照组采用自然光,处理组设置为16小时光照、8小时黑暗的光照制度。研究将20只雄性红翅䳍随机分为对照组(n=10)与人工光照组(n=10)。针对精子蛋白质组学检测,使用了10只个体的样本(其中6只来自对照组,4只来自人工光照组);由于对照组精浆样本体积不足,无法开展蛋白质组学检测,因此精浆蛋白质组学仅采用人工光照组样本制备的两个混合池进行分析,该部分分析仅为描述性研究。所有精子与精浆样本均以单独制备的方式处理,后续通过电喷雾电离四极杆飞行时间串联质谱(ESI Q-TOF MS/MS)完成蛋白质组学检测。




